Source: PRJNA350589⇩ HCoV-229E infection of the human HuH7 cell
line [Experiment B]
Wilhelm Lab, ECCPS, University of Giessen
The aim of the experiment is to identify
changes in host gene expression upon infection
of Huh7 hepatoma carcinoma cells with human
coronavirus HCoV-229E as compared to uninfected
cells and in comparison to cells stimulated
by IL-1alpha (10ng/ml) for 1h. All experiments
have been performed at 33°C which is required
for sufficient viral entry/replication.
Also included are two samples which have
been treated with a heat inactivated coronavirus
(56°C for 5min). Overall design: Single-color
hybridization of RNA from HuH7 cells. Duplicates.