Source:		PRJNA97445

     		Gene expression profiling of patients with
			severe acute respiratory syndrome (SARS)

			University Health Network (Owner)

			We used cDNA microarrays to systematically
			analyze host responses in SARS-infected individuals
			from onset of symptoms to discharge from
			hospital or death. We modeled gene expression
			in 60 datasets from 40 unique patients of
			varied clinical evolution with emphasis
			on correlating innate and adaptive immune
			responses with discrete clinical phases
			of the disease. Keywords: Comparative patient
			class comparision, SARS patients relative
			to healthy controls Overall design: Peripheral
			blood was collected in 5 ml heparinized
			blood collection tubes from study participants
			upon presentation to the hospital. The median
			length between onset of symptoms consistent
			with SARS and presentation to the hospital
			was 2 days (range 0-7 days). Peripheral
			blood specimens were then collected longitudinally
			every five days during hospitalization and
			at discharge from the hospital. Whole blood
			RNA was stabilized and purified using Paxgene
			Blood Collection Tubes and Paxgene RNA kits
			(Qiagen, Mississauga, ON, Canada). Sufficient
			RNA yields were obtained after Paxgene Whole
			Blood RNA isolation for 70 samples from
			40 unique SARS patients and 10 healthy controls
			(HC1-10) and amplified using MessageAmp
			kits (Ambion). Replicate measurements or
			dye swaps were not possible due to severe
			limitations in RNA quantity. All samples
			(Cy5) were hybridized against amplified
			reference RNA (Cy3) (Stratagene, La Jolla,
			CA). Longitudinal ANOVA were performed to
			select significantly altered genes in the
			pre-crisis (acute) phase of SARS (0-8 days
			since onset, n=22), crisis phase for non-severe
			SARS patients (8+ days since onset, n=15)
			and crisis phase for severe SARS patients
			(8+ days since onset, n=23).

			17537853 Interferon-mediated
			immunopathological events are associated with
			atypical innate and adaptive immune responses
			in patients with severe acute respiratory

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